Clinical StudyPublished: 2025-08

Multispectral Fluorescence Imaging for Sub-Millimeter Microvascular Assessment

Authors: Surgical Optics Research Division • Annals of Minimally Invasive Surgical Technology

Multispectral NIR fluorescence imaging in laparoscopic surgery

Peer-Reviewed Engineering Dossier

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Scientific Abstract

Executive Summary & Scope

“Evaluation of 4K narrow-band fluorescence overlay for real-time identification of ischemic margins in complex gastrointestinal resections.”

Methodology & Experimental Protocol

Evaluated dual-CMOS stereoscopic 4K imaging with simultaneous 785 nm laser excitation and 830 nm bandpass fluoroscopy. Real-time overlay metrics were benchmarked against histologically confirmed tissue perfusion boundaries in simulated surgical models.

Digital Object Identifier: 10.1097/sla.2025.11029Verified Publication

Quantitative Findings

Validated Study Outcomes

Sub-millimeter microvascular boundary discrimination at 60 frames per second

Zero chromatic aberration across combined visible (400-700nm) and NIR (700-900nm) bands

< 16 ms end-to-end video processing latency allowing continuous hand-eye coordination

Early intraoperative detection of hypoperfused tissue segments prior to resection

Clinical Significance

Validates that simultaneous true-color visible light and near-infrared fluorescence overlay provides surgeons with actionable real-time perfusion guidance without darkening the operative field.

Academic & Clinical Research Collaboration

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